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mom seqs
mothur > pcr.seqs(fasta=silva.bacteria.fasta, taxonomy=silva.bacteria.rdp.tax) ecoli The ecoli parameter is used to provide a fasta file containing a single reference sequence (e.g. for e. coli) this must be aligned. mothur will trim to the start and end positions of the reference sequence.

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pcr.seqs - mothur.org

mom seqs
mothur > summary.seqs(fasta=sogin.unique.align) Start End NBases Ambigs Polymer Minimum: 109 222 40 0 2 2.5%-tile: 4648 4875 57 0 3 25%-tile: 4655 4928 60 0 3 Median: 4655 4928 62 0 4 75%-tile: 4655 4928 65 0 4 97.5%-tile: 4655 4939 73 0 6 Maximum: 6793 6850 100 0 31 # of Seqs

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mom seqs
mothur > summary.seqs(fasta=sogin.unique.align) Start End NBases Ambigs Polymer Minimum: 109 222 40 0 2 2.5%-tile: 4648 4875 57 0 3 25%-tile: 4655 4928 60 0 3 Median: 4655 4928 62 0 4 75%-tile: 4655 4928 65 0 4 97.5%-tile: 4655 4939 73 0 6 Maximum: 6793 6850 100 0 31 # of Seqs: 21907

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